Journal: Frontiers in Immunology

Article Title: Integrative multi-omics reveals that downregulation of HLA-DPA1/DPB1 drives macrophage immune-metabolic dysregulation in pediatric asthma

doi: 10.3389/fimmu.2026.1835475

Figure Lengend Snippet: Experimental validation of HLA-DPA1 and HLA-DPB1 downregulation. (A) BEAS-2B human bronchial epithelial cells were treated with IL-13 (20 ng/mL) for 24 h to establish an in vitro asthma cell model. Quantitative real-time PCR analysis showed that the mRNA expression levels of HLA-DPA1, HLA-DPB1 and CIITA were significantly decreased in the IL-13-treated group compared with the control group. (B) BEAS-2B human bronchial epithelial cells were treated with IL-13 (20 ng/mL) for 24 h to establish an in vitro asthma cell model. Western blot analysis showed that the protein expression levels of HLA-DPA1, HLA-DPB1 and CIITA were significantly decreased in the IL-13-treated group compared with the control group. (C) PBMCs were collected from children with asthma and healthy controls. Quantitative qPCR analysis revealed that the mRNA expression levels of HLA-DPA1 and HLA-DPB1 were significantly lower in PBMCs from the asthma group compared with the healthy control group. (D) Western blot analysis was performed to assess the protein expression levels of HLA-DPA1 and HLA-DPB1 in PBMCs. The results demonstrated that the protein levels of both genes were markedly downregulated in the asthma group compared with the healthy control group. GAPDH was used as a loading control. *P<0.05; **P<0.01; ***P<0.001; ****P<0.0001, Student’s t-test, one-way ANOVA, post hoc comparisons, Tukey’s test.

Article Snippet: BEAS-2B (normal human bronchial epithelial) cells were purchased from ATCC and maintained in DMEM at 37 °C.

Techniques: Biomarker Discovery, In Vitro, Real-time Polymerase Chain Reaction, Expressing, Control, Western Blot